ꄴ前一个： 无
ꄲ后一个： 浙江省农业科学院

 

本项目牵头单位浙江大学动物科学学院，所在实验室为浙江省动物预防医学重点实验室和农业部动物病毒学重点实验室。本实验室／团队长期从事动物疫病和食源性病原生物学、微生物性食品安全领域的教学与研究：1）阐明食源性沙门氏菌跨种感染的分子机制；2）揭示食源性沙门菌优势菌型食物链传播分子基础；3）建立单增李斯特菌、副溶血弧菌等病原的分子溯源体系；4）发现单增李斯特菌中与抗酸应激相关的基因岛，探明其介导抗酸应激的代谢途径；建立单增李斯特菌在不同温度贮存条件下的生长模型和有机酸灭活模型；5）阐明副溶血弧菌的 VI 型分泌系统及其生物学功能；6）建立沙门氏菌、李斯特菌等重要病原的功能基因组学分析和研究平台；7）建构猪肉冷链流通质量安全控制技术和可追溯技术体系。获国家科技进步二等奖 1 项（2017），国家自然科学奖二等奖 1 项（2018），省部级科技奖励 2 项 （2019，2013），授权发明专利 6 件，近五年在 Nature 子刊等杂志上发表 SCI 论文50余篇。

浙江大学“双一流”平台拥有超过亿元大型仪器设备，包括IlluminaHiseq 2500，Nextseq 500 等基因组测序平台，布鲁克 MALDI-TOF 细菌鉴定质谱，Waters Q-tof 高分辨串联质谱仪等，具备开展微生物学、分子生物学、基因组学、蛋白组学、生物信息学等研究的良好平台。牵头团队近5年承担与病原微生物及相关检测技术课题包括国家科技重大专项、973 计划、863计划、行业专项、国家自然基金项目等；实验室建立了全国细菌耐药合作网络，拥有全国各地区分离的重要致病菌的菌种库，收集样本数3000余份，系统研究我国主要食源性致病菌的分子流行病学，以及重要致病菌感染机制和耐药机理等研究。实验室相关研究成果在 Nature 子刊等国际著名期刊上发表。

 

Expertise and role in the project:

Zhejiang University (ZJU) is a public research university ranked No. 3 in China, 16 in Asia by US News & World Report (2018), and 68 by QS World University Rankings (2019). ZJU consists of 36 colleges with a total student population of 54,000.  The two departments Veterinary Medicine and Food Sciences have infrastructure, well-equipped laboratories, software platforms, and active programs on microbial food safety with regular funding from the Ministry of Science &Technology and local governments since 2005. Dr. Yan Li and her colleagues have great expertise in studying microbial pathogens, bioinformatics and food safety, and are going to lead the project from the Chinese side with WP4 as the specific task focusing on implementation of novel omics methods of microbiological hazard detection.

Key persons involved (5 persons maximum):

Dr. Yan Li (F) is a principal investigator at ZJU. Her lab works on the zoonotic pathogens, including Salmonella, focusing on the genomic epidemiology and population diversity of foodborne transmission. Her lab led to 10 published papers, including Transboundary and Emerging Diseases, Clinical Infectious Diseases, etc. in the past 2 years.

Dr. Weihuan Fang (M) is an honored full professor at ZJU. His lab has been working on foodborne bacterial pathogens such as Listeria monocytogenes for decades and has had great expertise in biology of microbial pathogens, epidemiology and molecular genetics. He will act as a project coordinator between China and the EU.

Dr. Min Yue (M) joined ZJU as a principal investigator since Feb 2017. He focuses on bacterial genomics, and has published articles in high profile journals, including Nature Communications, Clinical Infectious Diseases, Journal of Antimicrobial Chemotherapy, 33 of which as the crossponding or first author.

Relevant publications, products, or services:

  1. 1.Detection of mcr-9-harbouring ESBL-producing Salmonella Newport isolated from an outbreak in a large-animal teaching hospital in the USA. Journal of Antimicrobial Chemotherapy 2020 DOI: 10.1093/jac/dkaa544.
  2. 2.Epidemiological investigation and antimicrobial resistance profiles of Salmonella isolated from breeder chicken hatcheries in Henan, China. Frontiers in Cellular and Infection Microbiology 2020. 10:497. DOI: 10.3389/fcimb.2020.00497.
  3. 3.The Application of Microbiome Culturomics in Veterinary Medicine. Acta Veterinaria et Zootechnica Sinica  2020. 51(12):2090-2101. DOI: 10.11843/j.issn.0366-6964.2020.12.004. In Chinese.
  4. 4.ONE Health Approach to Address Zoonotic Brucellosis: A Spatiotemporal Associations Study between Animals and Humans. Frontiers in Veterinary Science 2020. 7:521. DOI: 10.3389/fvets.2020.00521.
  5. 5.Characterization of Salmonella Resistome and Plasmidome in Pork Production System in Jiangsu, China. Frontiers in Veterinary Science 2020. 7:617. DOI: 10.3389/fvets.2020.00617.
  6. 6.Characterization of Multidrug Resistance Patterns of Salmonella enterica Serovar Rissen along the food chain in China. Antibiotics 2020. 9,660. DOI:10.3390/antibiotics9100660.
  7. 7.Prevalence and Antimicrobial Resistance of Salmonella Recovered from Pig-borne Food Products in Henan, China. Food Control 2020. 121: 107535 DOI: 10.1016/j.foodcont.2020.107535.
  8. 8.Genomic Characterization of Salmonella Uzaramo for Human Invasive Infection. Microbial Genomics 2020. 6(7): mgen000401 DOI: 10.1099/mgen.0.000401.
  9. 9.Genomic Characterization of mcr-1-carrying Salmonella enterica serovar 4,[5],12:i:-ST34 clone in China. Frontiers in Bioengineering and Biotechnology 2020. 8:663. DOI: 10.3389/fbioe.2020.00663.
  10. 10.Food Safety in China: Current Practices and Future Needs. Foodborne Pathogens and Disease 2020. 17(5):295. DOI: 10.1089/fpd.2020.29013.cfp.
  11. 11.Persistent Asymptomatic Human Infections by Salmonella Newport in China. mSphere 2020. 5:e00163-20. DOI: 10.1128/mSphere.00163-20.
  12. 12.Epidemiological and Genomic Characterization of Campylobacter jejuni Isolates from a Foodborne Outbreak at Hangzhou, China. International Journal of Molecular Sciences 2020. 21, 3001. DOI: 10.3390/ijms21083001.
  13. 13.The Study of Worldwide Brucella canis of Phylogenetic Group by Comparative Genomics-based Approaches. China Biotechnology 2020. 40(3):38-47. DOI: 10.13523/j.cb.1907053. In Chinese
  14. 14.Emerging colistin resistance in Salmonella enterica serovar Newport isolates from human infections. Emerging Microbes & Infections 2020. 9(1):535-538.
  15. 15.Genomic Characterization of New Variant of Hydrogen Sulfide H2S-Producing Escherichia coli with Multidrug Resistance Properties Carrying the mcr-1 Gene in China. Antibiotics 2020 13;9(2). pii: E80. DOI: 10.3390/antibiotics9020080.
  16. 16.An Updated Knowledge for Salmonella Type I Fimbriae. Chinese Journal of Preventive Veterinary Medicine 2020. 42(6): 527-533. DOI: 10.3969/j.issn.1008-0589.201907029. in Chinese
  17. 17.Global Burden of Colistin Resistant Bacteria: Mobilized Colistin Resistant Genes Study 1980-2018. Microorganisms 2020. 7(10), 461. DOI: 10.3390/microorganisms7100461.
  18. 18.Characterization of Salmonella Dublin isolated from Bovine and Human Hosts. BMC Microbiology 2020 19(1):226. DOI: 10.1186/s12866-019-1598-0.
  19. 19.PrsA contributes to Streptococcus suis serotype 2 pathogenicity by modulating secretion of selected virulence factors. Veterinary Microbiology 2019. 236:108375. DOI: 10.1016/j.vetmic.2019.07.027.
  20. 20.Emergence and Dissemination of mcr-Carrying Clinically Relevant Salmonella Typhimurium Monophasic Clone ST34. Microorganisms 2019. 28;7(9). pii: E298. DOI: 10.3390/microorganisms7090298.
  21. 21.Antibiotic resistance profiles of Salmonella recovered from finishing pigs and slaughter facilities in Henan, China. Frontiers in Microbiology 2020. 10:1513. DOI: 10.3389/fmicb.2019.01513.
  22. 22.Antibiotic Resistance in Salmonella Typhimurium Isolates Recovered From the Food Chain Through National Antimicrobial Resistance Monitoring System Between 1996 and 2016. Frontier in Microbiology 2019, 10:985. DOI: 10.3389/fmicb.2019.00985.
  23. 23.Antibiotic resistance in the “dark matter”. Clinical Infectious Diseases 2019. 69(2):379-380. DOI: 10.1093/cid/ciz007.
  24. 24.Diversified sources for human infections by Salmonella enterica serovar Newport. Transboundary and Emerging Diseases 2019. 66(2):1044-1048. DOI: 10.1111/tbed.13099.
  25. 25.Analysis of major human and foodborne pathogens and their resistance to antimicrobials in the USA in the past two decades: Implications for surveillance and control of antimicrobial resistance in China. Journal of Zhejiang University (Agriculture & Life Sciences) 2018. 44(2): 237-246. DOI: 10.3785/j.issn.1008-9209.2018.03.124. In Chinese
  26. 26.A Meta-Analysis of Major Foodborne Pathogens in Chinese Food Commodities Between 2006 and 2016. Foodborne Pathogens and Disease 2018, 15(4):187-197. DOI: 10.1089/fpd.2017.2417.
  27. 27.Modeling Transfer of Vibrio parahaemolyticus During Peeling of Raw Shrimp. Journal of Food Science 2018, 83(3):756-762. DOI: 10.1111/1750-3841.14064.
  28. 28.Multiple Food-Animal-Borne Route in Transmission of Antibiotic-Resistant Salmonella Newport to Humans. Frontiers in Microbiology 2018, 9:23. DOI: 10.3389/fmicb.2018.00023.
  29. 29.Bacterial Persistent Infection at the Interface between Host and Microbiota. Clinical Infectious Diseases 2017. 62(10):1325-6. DOI: 10.1093/cid/ciw136

Relevant projects related to SAFFI:

  1. 1.Molecular approach for investigating the foodborne bacterial transmission, National Program on Key Research Project of China, 2018.1-2021.12
  2. 2.Development of novel diagnostics and tracking toolkits for foodborne pathogens, National Program on Key Research Project, 2018.01-2020.12
  3. 3.Surveillance and control of major zoonotic pathogens from farm to slaughtering, Ministry of S&T, 2018.01-2020.12.
  4. 4.Control of Streptococcal infections in pigs, Ministry of Agriculture, 2013.01-2017.12.
  5. 5.Novel technologies of microbial toxins in foods. Ministry of S&T, 2012.01-2015.12.

Relevant infrastructure and equipment to be made available to SAFFI:

We have well-equipped microbiology and cell biology laboratories: homogenizers for DNA and RNA extraction, genomic sequencing, Real-time PCR thermo cyclers (Roche, ABI, etc.), full-wavelength spectrophotometer (Molecular Device), Regular thermo cyclers, series of high-speed centrifuges and ultracentrifuges (Eppendorf, Sorval, Beckman), Flow Cytometers (BD), High-performance computation servers etc. Software: house built-in galaxy platform for genomics analysis for the lab groups.